Geochemical baseline establishment and ecological risk evaluation of heavy metals in greenhouse soils from Dongtai,China

Currently, heavy metal (HM) contamination in greenhouse soils is a significant concern due to the rapid expansion of greenhouse agriculture. However, it is difficult to accurately assess HM pollution in greenhouse soils in China due to the lack of local geochemical baseline concentrations (GBCs) or corresponding background values. In the present study, the GBCs of HMs in Dongtai, a representative greenhouse area of China, were established from subsoils using cumulative frequency distribution (CFD) curves. The pollution levels of HMs and potential ecological risks were investigated using different quantitative indices, such as geo-accumulation index (I_geo), pollution index (PI), pollution load index (PLI) and ecological risk index (RI), based on these regional GBCs. The total concentrations of six metals (Cd, Cr, Cu, Ni, Pb and Zn) in surface soils were determined and shown to be lower than the concentrations reported in other greenhouse regions of China. The GBCs of Cd, Cr, Cu, Ni, Pb and Zn were 0.059–0.092, 39.20–54.50, 12.52–15.57, 20.63–23.26, 13.43–16.62 and 43.02–52.65 mg kg⁻¹, respectively. Based on this baseline criterion, Cd, Pb and Zn accumulated in the surface soils because they were present at concentrations higher than their baseline values. The soils were moderately polluted by Cd according to the I_geo values, and the PI results indicated that moderate Cd contamination was present in this area. The large variation of I_geo value of Cd revealed that Cd in this area was likely influenced by agricultural activities. The PLI showed that most of the study area was moderately polluted. However, an analysis of the RI showed that the investigated HMs had low ecological risks. Correlation analysis and principle component analysis suggested that the Cd, Pb and Zn in the greenhouse soils mainly originated from anthropogenic sources (agricultural activities, atmospheric deposition etc.), while Cr, Cu, and Ni originated from natural sources. The findings of this study illustrated the necessity of GBC establishment at the local scale to facilitate more accurate HM evaluation of greenhouse soils. It is advisable to pay more attention to Cd, which could cause environmental problems in the greenhouse system.     

Biotechnological applications of microbial bioconversions

Modern research has focused on the microbial transformation of a huge variety of organic compounds to obtain compounds of therapeutic and/or industrial interest. Microbial transformation is a useful tool for producing new compounds, as a consequence of the variety of reactions for natural products. This article describes the production of many important compounds by biotransformation. Emphasis is placed on reporting the metabolites that may be of special interest to the pharmaceutical and biotechnological industries, as well as the practical aspects of this work in the field of microbial transformations.     

Population dynamics of Macrophomina phaseolina in relation to disease management: A review

Macrophomina phaseolina (Tassi) Goid. causes seedling blight, charcoal rot, leaf blight, stem and pod rot on over 500 plant species in different parts of the world. The pathogen survives as sclerotia formed in host tissues which are released into the soil as tissue decay. Low soil moisture is considered the more important predisposing factor for M. phaseolina-induced diseases than high temperature. The intensity of the disease on a crop is related to the population of viable sclerotia in the soil and abiotic factors. The influence of various management strategies in reducing the number of viable propagules of the pathogen in the soil has been studied in order to minimize the impact of the disease. Any management approach that reduces inoculum density in the soil may reduce disease incidence on the host. However, to reduce inoculum density, quantitative determination of viable propagules from soil is necessary in order to understand the effect of management strategies on the population dynamics of this pathogen. Considerable work has been done on organic amendments, changing crop sequences with tolerant crops, fumigants, herbicides and tillage in managing M. phaseolina populations in the soil and the resulting disease. Solarization has been used in controlling M. phaseolina in different countries where this pathogen is causing disease on economically valuable crops. However, this method of soil disinfestation was effective in eliminating viable populations at the top soil layer although by combining other approaches its effectiveness was improved at lower soil depth. Use of biological control agents with or without organic amendments or after solarization has emerged to be a practical management approach in the control of M. phaseolina. In this paper, an attempt has been made to review those research findings where the influence of various management approaches on survival of M. phaseolina mainly sclerotia have been investigated.     

Amyloid by Design: Intrinsic Regulation of Microbial Amyloid Assembly

The term amyloid has historically been used to describe fibrillar aggregates formed as the result of protein misfolding and that are associated with a range of diseases broadly termed amyloidoses. The discovery of “functional amyloids” expanded the amyloid umbrella to encompass aggregates structurally similar to disease-associated amyloids but that engage in a variety of biologically useful tasks without incurring toxicity. The mechanisms by which functional amyloid systems ensure nontoxic assembly has provided insights into potential therapeutic strategies for treating amyloidoses. Some of the most-studied functional amyloids are ones produced by bacteria. Curli amyloids are extracellular fibers made by enteric bacteria that function to encase and protect bacterial communities during biofilm formation. Here we review recent studies highlighting microbial functional amyloid assembly systems that are tailored to enable the assembly of non-toxic amyloid aggregates.     

Inhibitory effect of aspirin on cholera toxin-induced phospholipase and cyclo-oxygenase activity

Cholera toxin (CT) stimulated phospholipase activity and caused [3H]arachidonic acid (3H-AA) release in a murine macrophage/monocyte cell line. Pretreatment of cells with dexamethasone, a phospholipase A2 (PLA2) inhibitor, did not affect CT-induced 3H-AA release. In contrast, aspirin, which is an inhibitor of phospholipase C (PLC), blocked CT-induced 3H-AA release and subsequent prostaglandin (PC) synthesis. The inhibitory effect of aspirin was dose dependent, with 4 mM reducing the CT response by approximately 50%. Similarly, inhibition was time dependent, occurring when the drug was added to the culture medium as late as 30 min after CT. Brief exposure (30 min) of the cells to aspirin did not alter their subsequent response to CT, but 3H-AA release from cells exposed to aspirin for 2.5 h was irreversibly inhibited. The data suggested that CT stimulation of AA metabolism may involve increased PLC activity.     

North and South: Exploring isotopic analysis of bone carbonates and collagen to understand post-medieval diets in London and northern England

Objectives

We evaluate the potential of paired isotopic analysis of bone carbonate and collagen to examine the diet of post-medieval human and animal populations from England (17th–19th c.), including, for the first time, manufacturing towns in northern England. The potential for identifying C₄ crop consumption is explored alongside regional and local patterning in diet by sex and socioeconomic status.

Materials and Methods

Humans (n = 216) and animals (n = 168) were analyzed from sites in London and northern England for both carbon and nitrogen isotopes of bone collagen (𝛿¹³C_coll, 𝛿¹⁵N_coll). Isotopic analysis of bone carbonates (𝛿¹³C_carb, 𝛿¹⁸O_carb) was carried out on all humans and 27 animals, using Fourier transform infrared spectroscopy–attenuated total reflectance to assess diagenesis.

Results

Variations in diet were observed between and within different populations by geographical location and socioeconomic status. Three pigs and one cow consumed C₄ resources, indicating the availability of C₄-fed animal protein. Londoners consumed more animal and marine protein and C₄ resources. Middle- and upper-class populations from both London and northern populations also had greater access to these foods compared to those of lower status in the same regions.

Discussion

This substantial multi-isotope dataset deriving from bone carbonate and collagen combined from diverse post-medieval urban communities enabled, for the first time, the biomolecular identification of the dynamics of C₄ consumption (cane sugar/maize) in England, providing insight into the dynamics of food globalization during this period. We also add substantially to the animal dataset for post-medieval England, providing further insight into animal management during a key moment of agricultural change.

     

Hydraulic acclimation in a Mediterranean oak subjected to permanent throughfall exclusion results in increased stem hydraulic capacitance

Stem water storage capacity and hydraulic capacitance (C_S) play a crucial role in tree survival under drought-stress. To investigate whether C_S adjusts to increasing water deficit, variation in stem water content (StWC) was monitored in vivo for 2 years and related to periodical measurements of tree water potential in Mediterranean Quercus ilex trees subjected either to permanent throughfall exclusion (TE) or to control conditions. Seasonal reductions in StWC were larger in TE trees relative to control ones, resulting in greater seasonal C_S (154 and 80 kg m⁻³ MPa⁻¹, respectively), but only during the first phase of the desorption curve, when predawn water potential was above −1.1 MPa. Below this point, C_S decreased substantially and did not differ between treatments (<20 kg m⁻³ MPa⁻¹). The allometric relationship between tree diameter and sapwood area, measured via electrical resistivity tomography, was not affected by TE. Our results suggest that (a) C_S response to water deficit in the drought-tolerant Q. ilex might be more important to optimize carbon gain during well-hydrated periods than to prevent drought-induced embolism formation during severe drought stress, and (b) enhanced C_S during early summer does not result from proportional increases in sapwood volume, but mostly from increased elastic water.     

Respiration and growth of tomato fruit

The respiration rate and diameter expansion growth of young tomato fruit were measured simultaneously and related to changes in carbon import and plant water status. Respiration rate was directly proportional to the volume expansion rate of fruit growing on isolated plant tops at a positive water potential, whether the growth rate was changed by changing the fruit temperature or by manipulating the source:sink ratio of the plants. From the latter relationship, the maintenance respiration rate was estimated by extrapolation to zero growth and was found to be about 25% of the respiration rate of the average fruit at 21°C. Alternatively, when carbon import was prevented by heat-ringing the fruit peduncle, the respiration rate of the fruit declined to about 40% of the control rate and remained steady, while the expansion rate then declined steadily to >10% of the control rate. These results show that fruit expansion was not contributing significantly to fruit respiration. Indeed, large fluctuations in fruit expansion rate could also be induced by repeated darkening and illumination of potted plants without a corresponding change in fruit respiration. Most significantly, fruit expansion was considerably reduced when plants were allowed to wilt, hut there was no change in fruit respiration rate unless the fruit peduncle was subsequently heat-ringed. We conclude that a major part of the respiration of young tomato fruit was determined by the rate of carbon import, or associated processes, and that fruit expansion per se can occur with relatively low respiratory costs.     

Effects of exotic plant invasion on soil nitrogen availability

外来植物入侵对土壤氮循环和氮有效性的影响是入侵成功或进一步加剧的重要原因。通过对比相同研究地点入侵区域和无入侵区域的土壤原位氮状态差异, 探讨了外来植物入侵对土壤氮有效性的影响程度和生理生态学机制。基于107篇相关研究文献数据的整合, 发现植物入侵区域相对于无入侵区域土壤总氮、铵态氮、硝态氮、无机氮、微生物生物量氮含量显著增加, 增幅分别为(50 ± 14)%、(60 ± 24)%、(470 ± 115)%、(69 ± 25)%、(54 ± 20)%。土壤硝态氮含量增幅较大反映硝化作用增强, 这可能增加入侵植物硝态氮利用以及喜硝植物的共存。温带地区植物入侵后土壤的硝态氮含量增幅显著高于亚热带地区。固氮植物入侵后土壤的总氮和无机氮含量增幅均显著高于非固氮植物入侵。木本和常绿植物入侵后土壤的总氮含量增幅分别高于草本和落叶植物入侵; 而土壤铵态氮含量的增幅没有显著差异且与固氮入侵植物占比无明显关系; 然而硝态氮含量的增幅普遍较高且与固氮入侵植物占比显著正相关。外来入侵植物固氮功能以及凋落物质量和数量是影响土壤氮矿化和硝化过程的关键因素。该研究为理解外来植物入侵成功和加剧的机制以及入侵植物功能性状与土壤氮动态之间的关系提供了新的见解。